WebJan 14, 1999 · Two recent papers [1], [2] have now reported a series of experiments aimed at characterizing the mobility mechanism of a bacterial group II intron. The results support an elegantly simple model, in which the intron RNA integrates into the target DNA by reverse splicing and then serves as the template for the synthesis of complementary DNA. WebGroup II introns are a large class of self-catalytic ribozymes and mobile genetic elements found within the genes of all three domains of life. Ribozyme activity (e.g., self- splicing) can occur under high-salt conditions in vitro. However, assistance from proteins is required for in vivo splicing. [1]
Mechanisms of intron gain and loss in Drosophila - Academia.edu
WebMar 15, 2005 · Function. Multifunctional protein that promotes group II intron splicing and mobility by acting both on RNA and DNA. It has three activities: reverse transcriptase (RT) for intron duplication, maturase to promote splicing, and DNA endonuclease for site-specific cleavage of recipient alleles. The intron-encoded protein promotes splicing by ... WebIt now provides a structural foundation for addressing major questions about the reverse transcription mechanisms of group II intron and other non-LTR-retroelement RTs, the evolution of RTs, and the further engineering … instow devon pubs
Structure of a group II intron in complex with its reverse ...
WebInduro Reverse Transcriptase is a group II intron-encoded RT that exhibits high processivity, increased thermostability, and increased tolerance of inhibitors in the synthesis of cDNA from RNA. It is an ideal enzyme for challenging cDNA synthesis from long … WebE. r. Group II intron reverse transcriptase Accession ID: CBK92290.1 Source: Eubacterium rectale ... Crystal structures of a group II intron maturase reveal a missing link in spliceosome evolution. Nat Struct Mol Biol. 2016 Jun;23(6):558-65. Zhao C, Liu F, Pyle AM. An ultraprocessive, accurate reverse transcriptase encoded by a metazoan group ... WebMar 30, 2024 · Group II introns aI1 and aI2 of the yeast mitochondrial COXI gene are mobile elements that encode an intron-specific reverse transcriptase (RT) activity. We show here that the introns of Saccharomyces cerevisiae ID41-6/161 insert site specifically into intronless alleles. The mobility is accompanied by efficient, but highly asymmetric, … joanne atha