Bodipy fatty acid
WebNov 9, 2016 · Saturated fatty acid (SFA)-related lipotoxicity is a pathogenesis of diabetes-related renal proximal tubular epithelial cell (PTEC) damage, closely associated with a progressive decline in renal function. This study was designed to identify a free fatty acid (FFA) metabolism-related enzyme that can protect PTECs from SFA-related lipotoxicity. … WebFeb 2, 2024 · We chose to use Bodipy-palmitate as fatty acids of the length (16 carbons) are more commonly found in the membranes of macrophages infected with Mtb. For labeling one T75 flask add 1.6 ml of 100 μM Bodipy-palmitate in 1% fatty acid-free BSA (from …
Bodipy fatty acid
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WebApr 28, 2006 · The BODIPY-labeled fatty acid analogues are a useful addition to the tools employed to study the cellular uptake and metabolism of lipids. In this study, we show that BODIPY FL C16 binds to purified liver and intestinal fatty acid-binding proteins with high … WebThe green fluorescent fatty acid, BODIPY™ FL C16 can be used as a synthetic precursor to a wide variety of fluorescent phospholipids. Unlike fluorophores like pyrene, DPH and NBD, BODIPY™ dyes are relatively environment insensitive and is fluorescent in both aqueous and lipid environments.
WebLipid body formation in the alga was visualized through BODIPY 505/515 staining and fluorescence microscopy. The fatty acid methyl ester (FAME) production level of KNM0029C was 178.6 mg L(-1) culture and 2.3-fold higher than that of C. reinhardtii CC-125 at 4 °C. Analysis of the FAME content showed a predominance of polyunsaturated fatty … WebJul 21, 1998 · The fifth, fatty acid transport protein (FATP), was identified by Schaffer and Lodish (13) using an expression-cloning strategy. FATP is a 63-kDa plasma membrane protein which increases LCFA uptake when stably expressed in cell lines but has no …
WebApr 14, 2024 · To specifically test for fatty acid uptake, we labelled the cells with the saturated lipid probe C1-Bodipy 500/510 C12 and showed that MPI KO cells significantly increased fatty acid uptake (Fig ... WebThe QBT Fatty Acid Uptake Assay uses a BODIPY®-dodecanoic acid fluorescent fatty acid analog coupled with our proprietary quench technology. The BODIPY label provides an ideal long chain fatty acid analog that behaves much like natural fatty acids and is a …
WebApr 28, 2016 · While the human placenta must provide selected long-chain fatty acids to support the developing fetal brain, little is known about the mechanisms underlying the transport process. We tracked the movement of the fluorescently labeled long-chain fatty …
WebJun 24, 2024 · In order to confirm that SSO indeed blocked fatty acid uptake, the uptake of 4, 4-difluoro-5, 7-dimethyl-4-bora-3a,4a-diaza- s -indacene-3-dodecanoic acid (BODIPY TM FL C12), a fluorescent fatty acid analog, in the presence of SSO in BV2 cells was examined by flow cytometry. player evaluation form baseballWebMay 9, 2024 · Indeed, recent studies using BODIPY 558/568 C 12, in which a BODIPY dye is appended to the ω-terminus of lauric acid, revealed how FAs are supplied to mitochondria from TAGs in lipid droplets... primary key rid uidprimary key requiredWebJun 1, 2007 · Bodipy FL c16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid) is an exogenous indicator that has been successfully and extensively used in vitro to measure fatty... primary key relationshipWebJul 21, 1998 · They were then washed three times with PBS/fetal calf serum for 5 min followed by an incubation for 2 min at 37°C in fatty acid uptake solution, which contained 0.1 μM BODIPY-FA and 0.1% fatty acid-free BSA in PBS (13). After 2 min, the cells were washed four times with ice-cold PBS/0.1% BSA. primary key related fieldWebcently labeled fatty acid analogs, particularly C1-BODIPY-C12, have proved to be suitable alternatives to radiolabeled LCFAs. Uptake kinetics of C1-BODIPY-C12 have been determined for several cell types including adipocytes (Fig. 7.1) and the fluorescent fatty … primary key ridWebBorondipyrromethene (BODIPY) dyes are bright and photostable fluorophores. BDP FL is a dye for fluorescein (FAM) channel. This is a free unactivated carboxylic acid derivative of BDP FL. This reagent is useful as a non-reactive control, for reference, and calibration. It … player experience and need satisfaction scale