WebAE, absorptive enterocyte; B, BODIPY; B-C4, B-C6, B-C8, B-C12, B-C16, BODIPY–fatty acids; FA, fatty acid; TAG, triglyceride. Techniques Used: In Vitro, Fluorescence, Incubation. Figure Legend Snippet: Etomoxir reduces fatty acid export. ( A ) Left : Schematic showing basal reservoir sampling after application of B–-fatty acids to the apical ... WebArticle Snippet: For detecting FA uptake, 10 µM of the fluorescent long-chain FA analog BODIPY™ 500/510 C1, C12 ( ThermoFisher Scientific) was loaded into isolated cardiomyocytes for 5 min, and the cells were then washed with 0.5% Bovine serum albumin (BSA) in PBS twice. Techniques: Western Blot
Invitrogen BODIPY 500/510 C1, C12 (4,4-Difluoro-5-Methyl-4-Bora …
WebBODIPY 558/568 C12 is a fatty acid-conjugated fluorescent probe for lipid droplets. 1, 2 It displays excitation/emission maxima of 558/568 nm, respectively, and has been used to monitor the localization and dynamics of lipid droplets in live cells. WARNING This product is not for human or veterinary use. sen broyhill
Renal Purge of Hemolymphatic Lipids Prevents the ... - ScienceDirect
WebThe green fluorescent BODIPY FL C12 fatty acid can be used as a synthetic precursor to a wide variety of fluorescent phospholipids. BODIPY dyes are relatively environment insensitive and is fluorescent in both aqueous and lipid environments with sharp emission peak. Calculators Common stock solution preparation Table 1. WebJun 1, 2016 · The effect of GSK650394 on FA uptake was quantified by measuring intracellular accumulation of fluorescent FA (C1-BODIPY®-C12). Results Exposure to acute or chronic cycling hypoxia was associated with up-regulated expression of SGK1 in NCI-H460 cells, increased uptake of FA from the culture medium, and increased sensitivity to … WebThe BODIPY fluorophores have spectral characteristics that are often superior to those of fluorescein, tetramethylrhodamine, Texas Red and longer-wavelength dyes. With derivatives that span the visible spectrum ( Figure 1.4.1 ), BODIPY dyes are extremely versatile. sen bray indiana